Journal: Frontiers in Immunology

Article Title: MIF tautomerase inhibitor TE-11 prevents inflammatory macrophage activation and glycolytic reprogramming while reducing leukocyte migration and improving Crohn’s disease-like colitis in male mice

doi: 10.3389/fimmu.2025.1558079

Figure Lengend Snippet: TE-11 alleviates the migratory responsiveness of human neutrophils and eosinophils towards MIF and major chemoattractants. (A, B) Polymorphonuclear leukocytes (PMNL) and (C, D) purified eosinophils were pretreated with TE-11 (20 µM) at 37°C for 30 min and were allowed to migrate towards (A, C) MIF (3 nM, n = 7-8), (B) IL-8 (10 nM, n = 6) or (D) CCL11 (10 nM, n = 7) in a micro-Boyden chamber at 37°C for 60 min. Migrated cells were enumerated by flow cytometry on a BD Canto II flow cytometer (acquisition set for 30 sec at medium flow rate) and expressed as % of the respective mean control response (MIF, IL-8 or CCL11 alone). Data are shown as mean ± SD of indicated independent experiments. Paired t-test or Wilcoxon test, *P < 0.05. All experiments were performed in technical triplicates. MIF, macrophage migration inhibitory factor .

Article Snippet: Cells were resuspended in assay buffer (PBS with 0.9 mmol/L Ca 2+ and 0.5 mmol/L Mg 2+ , supplemented with 0.1% BSA, 10 mmol/L HEPES, and 10 mmol/L glucose, pH 7.4), pretreated with TE-11 (20 μM) for 30 min at 37°C and allowed to migrate towards MIF (3 nM; Peprotech, London, UK; eosinophils: n = 7, neutrophils: n = 8), IL-8 (10 nM; Immunotools, Friesoythe, DE; neutrophils: n = 6) or CCL11 (10 nM; Immunotools, Friesoythe, DE; eosinophils: n = 7) for another 60 min at 37°C in a 48-well micro-Boyden chamber using PVP-free polycarbonate filters with a pore size of 5 μm (eosinophils) or 3 μm (neutrophils) (Sterlitech, Auburn, US).

Techniques: Purification, Flow Cytometry, Control, Migration